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filter top polystyrene flow cytometry tube  (Fisher Scientific)
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Fisher Scientific filter top polystyrene flow cytometry tube
A: Schematic overview of the screening approach. hiPSC-derived CD34 + hemogenic endothelial cells were differentiated towards ProT cells for 14 days in a stroma-free protocol . At iT day 14 cells were harvested and replated into a 384 well plate and exposed to a small molecule library. After 7 days cells were stained with anti CD56, anti CD4, anti CD7 with 7-AAD and Calcein Violet viability dyes. Scale bar indicates 20 µm. B: Primary screening results. 5 hits were identified when a Z-score threshold of 1 was applied. C: Hit validation, bar graphs depicting mean percentage of CD4 + cells out of CD45 + population on iT day 28 in hiPSC line EZH1 mut/wt (screening line), BCH1157 and BCH1566. Error bars depict SEM of replicate wells, one way ANOVA comparison against DMSO. E: Exemplary flow <t>cytometry</t> plots on iT day 28 demonstrating an increased proportion of CD4 + CD8 + DP cells after GNF351 treatment in BCH1157 hiPSC-derived ProT cells. F: Mean values plotted from two independent experiments with either continuous drug treatment (solid circles) or one time drug exposure (open circles). SEM of technical replicates is indicated by blue shading. Two-way ANOVA with Dunnett’s multiple comparison test against respective untreated cells.
Filter Top Polystyrene Flow Cytometry Tube, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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filter top polystyrene flow cytometry tube - by Bioz Stars, 2026-05
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A: Schematic overview of the screening approach. hiPSC-derived CD34 + hemogenic endothelial cells were differentiated towards ProT cells for 14 days in a stroma-free protocol . At iT day 14 cells were harvested and replated into a 384 well plate and exposed to a small molecule library. After 7 days cells were stained with anti CD56, anti CD4, anti CD7 with 7-AAD and Calcein Violet viability dyes. Scale bar indicates 20 µm. B: Primary screening results. 5 hits were identified when a Z-score threshold of 1 was applied. C: Hit validation, bar graphs depicting mean percentage of CD4 + cells out of CD45 + population on iT day 28 in hiPSC line EZH1 mut/wt (screening line), BCH1157 and BCH1566. Error bars depict SEM of replicate wells, one way ANOVA comparison against DMSO. E: Exemplary flow cytometry plots on iT day 28 demonstrating an increased proportion of CD4 + CD8 + DP cells after GNF351 treatment in BCH1157 hiPSC-derived ProT cells. F: Mean values plotted from two independent experiments with either continuous drug treatment (solid circles) or one time drug exposure (open circles). SEM of technical replicates is indicated by blue shading. Two-way ANOVA with Dunnett’s multiple comparison test against respective untreated cells.

Journal: bioRxiv

Article Title: Efficient Generation of Functional TCRαβ + Cytotoxic T Cells from hiPSCs via Small-Molecule Modulation

doi: 10.64898/2026.03.31.715684

Figure Lengend Snippet: A: Schematic overview of the screening approach. hiPSC-derived CD34 + hemogenic endothelial cells were differentiated towards ProT cells for 14 days in a stroma-free protocol . At iT day 14 cells were harvested and replated into a 384 well plate and exposed to a small molecule library. After 7 days cells were stained with anti CD56, anti CD4, anti CD7 with 7-AAD and Calcein Violet viability dyes. Scale bar indicates 20 µm. B: Primary screening results. 5 hits were identified when a Z-score threshold of 1 was applied. C: Hit validation, bar graphs depicting mean percentage of CD4 + cells out of CD45 + population on iT day 28 in hiPSC line EZH1 mut/wt (screening line), BCH1157 and BCH1566. Error bars depict SEM of replicate wells, one way ANOVA comparison against DMSO. E: Exemplary flow cytometry plots on iT day 28 demonstrating an increased proportion of CD4 + CD8 + DP cells after GNF351 treatment in BCH1157 hiPSC-derived ProT cells. F: Mean values plotted from two independent experiments with either continuous drug treatment (solid circles) or one time drug exposure (open circles). SEM of technical replicates is indicated by blue shading. Two-way ANOVA with Dunnett’s multiple comparison test against respective untreated cells.

Article Snippet: The cells were then filtered through a filter-top polystyrene flow cytometry tube (Fisher Scientific, #08-771-23), washed with flow cytometry buffer (PBS + 2% FBS + antibiotics), and centrifuged at 300xg for 5 minutes followed by antibody staining and flow cytometric analysis for expression of CD45 and CD19.

Techniques: Derivative Assay, Staining, Biomarker Discovery, Comparison, Flow Cytometry